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KMID : 0603820130190040310
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 4 p.310 ~ p.314
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Polymorphisms of Cytochrome P450 2E1 Gene in Korean Patients with Renal Failure
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Yoo Min
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Abstract
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CYP2E1 in the liver has been studied intensively because it is involved in the metabolic activation of xenobiotics. It is inducible by alcohol, so it has been suspected as the cause of cancer in the stomach and lung. The possible role of CYP2E1 has been suggested strongly as causing tissue damage in mice with renal failure. It was also suspected that 5"-flanking region of CYP2E1 gene might be involved with renal failure. So, we investigated polymorphism of restriction enzyme sites within CYP2E1 gene using the PCR-RFLP analysis. PstI and RsaI sites were located at 5"-flanking region and DraI site was located at intron 6. All three types (W/W, W/S, S/S) were observed for these enzymes although each incidence was somewhat different depending the enzyme sites. W/W was prominent for PstI whereas W/S was markedly high for RsaI. Overall, polymorphic incidence in patients was somewhat higher than normal population. This research should facilitate further investigation of CYP2E1 at genetic level as the direct cause of tissue damage in various organs.
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KEYWORD
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CYP2E1, Alcohol, Polymorphism, Tissue damage, Renal failure
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